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TET1‐Mediated Oxidation of 5‐Formylcytosine (5fC) to 5‐Carboxycytosine (5caC) in RNA
Author(s) -
BasantaSanchez Maria,
Wang Rui,
Liu Zhenzhen,
Ye Xiaohan,
Li Minyong,
Shi Xiaodong,
Agris Paul F.,
Zhou Yubin,
Huang Yun,
Sheng Jia
Publication year - 2017
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201600328
Subject(s) - rna , chemistry , biochemistry , gene
It was recently revealed that 5‐methylcytosine (5mC) in mRNA, similar to its behavior in DNA, can be oxidized to produce 5‐hydroxymethylcytosine (5hmC) and 5‐formylcytosine (5fC), implying the potential regulatory roles of this post‐transcriptional RNA modification. In this study, we demonstrate the in vitro oxidation of 5fC to 5‐carboxycytidine (5caC) by the catalytic domain of mammalian ten‐eleven translocation enzyme (TET1) in different RNA contexts. We observed that this oxidation process has very low sequence dependence and can take place in single‐stranded, double‐stranded, or hairpin forms of RNA sequences, although the overall conversion yields are low.