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Cover Picture: Multiplexed Imaging of Protein Phosphorylation on Membranes Based on Ti IV Functionalized Nanopolymers (ChemBioChem 10/2016)
Author(s) -
Iliuk Anton,
Li Li,
Melesse Michael,
Hall Mark C.,
Tao W. Andy
Publication year - 2016
Publication title -
chembiochem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201600249
Subject(s) - phosphorylation , protein phosphorylation , blot , chemistry , signal transduction , membrane , membrane protein , antibody , biochemistry , microbiology and biotechnology , computational biology , biology , protein kinase a , gene , immunology
The cover picture shows that the accurate determination of protein phosphorylation uncovers dynamic cellular signaling events that are not evident from protein expression levels. The most dominant biochemical assay, western blotting, is limited by inadequate availability and the poor quality of phospho‐specific antibodies for phosphorylated proteins. Multiplexed assays based on antibodies are further constrained by steric interference between the antibodies. Soluble nanopolymer, pIMAGO, functionalized with Ti IV ions for chelating phosphoproteins in high specificity and with infrared fluorescent tags, is capable of universally detecting phosphoproteins. In combination with regular antibodies, this allows for multiplexed imaging and accurate determination of protein phosphorylation on membrane. We anticipate broad applications of this new strategy in monitoring cellular signaling pathways and discovering new signaling events. More information can be found in the communication by W. A. Tao et al. on page 900 in Issue 10, 2016 (DOI: 10.1002/cbic.201600068).