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Position‐Specific Mass Shift Analysis: A Systematic Method for Investigating the EI‐MS Fragmentation Mechanism of epi ‐Isozizaene
Author(s) -
Rabe Patrick,
Klapschinski Tim A.,
Dickschat Jeroen S.
Publication year - 2016
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201600237
Subject(s) - fragmentation (computing) , mechanism (biology) , mass spectrometry , chemistry , position (finance) , computational chemistry , physics , chromatography , computer science , finance , quantum mechanics , economics , operating system
The EI‐MS fragmentation mechanism of the bacterial sesquiterpene epi ‐isozizaene was investigated through enzymatic conversion of all 15 synthetic ( 13 C 1 )FPP isotopomers with the epi ‐isozizaene synthase from Streptomyces albus and GC‐MS and GC‐QTOF analysis including MS‐MS. A systematic method, which we wish to call position‐specific mass shift analysis, for the identification of the full set of fragmentation reactions was developed.