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Bioorthogonal Chemical Reporters for Monitoring Unsaturated Fatty‐Acylated Proteins
Author(s) -
Thi Emmanuelle,
Percher Avital,
Hang Howard C.
Publication year - 2016
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201600213
Subject(s) - bioorthogonal chemistry , biochemistry , chemistry , fatty acid , proteomics , oleic acid , covalent bond , chemical biology , fatty acid binding protein , unsaturated fatty acid , oleosin , organic chemistry , click chemistry , gene
Dietary unsaturated fatty acids, such as oleic acid, have been shown to be covalently incorporated into a small subset of proteins, but the generality and diversity of this protein modification has not been studied. We synthesized unsaturated fatty‐acid chemical reporters and determined their protein targets in mammalian cells. The reporters can induce the formation of lipid droplets and be incorporated site‐specifically onto known fatty‐acylated proteins and label many proteins in mammalian cells. Quantitative proteomics analysis revealed that unsaturated fatty acids modify similar protein targets to saturated fatty acids, including several immunity‐associated proteins. This demonstrates that unsaturated fatty acids can directly modify many proteins to exert their unique and often beneficial physiological effects in vivo.