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Multiplexed Imaging of Protein Phosphorylation on Membranes Based on Ti IV Functionalized Nanopolymers
Author(s) -
Iliuk Anton,
Li Li,
Melesse Michael,
Hall Mark C.,
Tao W. Andy
Publication year - 2016
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201600068
Subject(s) - phosphorylation , blot , membrane , protein phosphorylation , antibody , protein detection , chemistry , membrane protein , biochemistry , western blot , microbiology and biotechnology , biology , protein kinase a , nanotechnology , materials science , gene , immunology
Accurate protein phosphorylation analysis reveals dynamic cellular signaling events not evident from protein expression levels. The most dominant biochemical assay, western blotting, suffers from the inadequate availability and poor quality of phospho‐specific antibodies for phosphorylated proteins. Furthermore, multiplexed assays based on antibodies are limited by steric interference between the antibodies. Here we introduce a multifunctionalized nanopolymer for the universal detection of phosphoproteins that, in combination with regular antibodies, allows multiplexed imaging and accurate determination of protein phosphorylation on membranes.