Premium
Phosphoramidate Ligation of Oligonucleotides in Nanoscale Structures
Author(s) -
Kalinowski Matthäus,
Haug Rüdiger,
Said Hassan,
Piasecka Sylwia,
Kramer Markus,
Richert Clemens
Publication year - 2016
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201600061
Subject(s) - phosphoramidate , oligonucleotide , dna origami , chemical ligation , dna , covalent bond , context (archaeology) , chemistry , nucleotide , combinatorial chemistry , ligation , steric effects , template , dna nanotechnology , nanotechnology , stereochemistry , peptide , biochemistry , materials science , biology , organic chemistry , paleontology , microbiology and biotechnology , gene
The folding of long DNA strands into designed nanostructures has evolved into an art. Being based on linear chains only, the resulting nanostructures cannot readily be transformed into covalently linked frameworks. Covalently linking strands in the context of folded DNA structures requires a robust method that avoids sterically demanding reagents or enzymes. Here we report chemical ligation of the 3′‐amino termini of oligonucleotides and 5′‐phosphorylated partner strands in templated reactions that produce phosphoramidate linkages. These reactions produce inter‐nucleotide linkages that are isoelectronic and largely isosteric to phosphodiesters. Ligations were performed at three levels of complexity, including the extension of branched DNA hybrids and the ligation of six scaffold strands in a small origami.