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Color‐Coded Super‐Resolution Small‐Molecule Imaging
Author(s) -
Beuzer Paolo,
La Clair James J.,
Cang Hu
Publication year - 2016
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201600013
Subject(s) - super resolution microscopy , superresolution , resolution (logic) , microscopy , small molecule , dna , biophysics , nanotechnology , optical imaging , computational biology , chemistry , biology , computer science , physics , optics , materials science , biochemistry , artificial intelligence , image (mathematics) , scanning confocal electron microscopy
Although the development of super‐resolution microscopy dates back to 1994, its applications have been primarily focused on visualizing cellular structures and targets, including proteins, DNA and sugars. We now report on a system that allows both monitoring of the localization of exogenous small molecules in live cells at low resolution and subsequent super‐resolution imaging by using stochastic optical reconstruction microscopy (STORM) on fixed cells. This represents a powerful new tool to understand the dynamics of subcellular trafficking associated with the mode and mechanism of action of exogenous small molecules.