Premium
Cover Picture: Detection of Human α‐ L ‐Fucosidases by a Quinone Methide‐Generating Probe: Enhanced Activities in Response to Helicobacter pylori Infection (ChemBioChem 11/2015)
Author(s) -
Nandakumar Manjula,
Hsu YuLing,
Lin Jason C.Y,
Lo Chun,
Lo LeeChiang,
Lin ChunHung
Publication year - 2015
Publication title -
chembiochem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201590028
Subject(s) - chemistry , quinone methide , enzyme , biochemistry , context (archaeology) , cleavage (geology) , quinone , stereochemistry , biology , paleontology , fracture (geology)
The cover picture shows a quinone methide‐generating, activity‐based probe that has been applied for detecting increases in the activity of human fucosidase in gastric epithelial cells after infection with Helicobacter pylori. The enzyme's activity is associated with several diseases. Upon reaction with human fucosidase, this probe undergoes glycosidic cleavage and subsequent 1,4‐elimination to generate a reactive quinone methide that reacts with nearby nucleophile(s) from the enzyme, thereby forming a BODIPY‐labeled enzyme adduct. In their report on p. 1555 ff., L.‐C. Lo, C.‐H. Lin et al. shown how the probe is useful for examining the presence, activity, and localization of human fucosidase in vivo in the context of bacterial infection. They further report their studies on the effect of several bacterial stimulants on this enhanced enzyme activity, and their identification of lipopolysaccharides as a major contributing factor.