Premium
An Efficient Lanthanide‐Dependent DNAzyme Cleaving 2′–5′‐Linked RNA
Author(s) -
Zhou Wenhu,
Ding Jinsong,
Liu Juewen
Publication year - 2016
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201500690
Subject(s) - deoxyribozyme , lanthanide , rna , chemistry , cofactor , biochemistry , combinatorial chemistry , stereochemistry , dna , ion , enzyme , organic chemistry , gene
Abstract RNA can form two types of linkage. In addition to the predominant 3′–5′ linkage, 2′–5′‐linked RNA is also important in biology, medicine, and prebiotic studies. Here, in vitro selection was used to isolate a DNAzyme that specifically cleaves 2′–5′ RNA by using Ce 3+ as the metal cofactor, but leaves the 3′–5′ counterpart intact. This Ce5 DNAzyme requires trivalent light lanthanide ions and shows a rate of 0.16 min −1 in the presence of 10 μ m Ce 3+ ; the activity decreases with heavier lanthanide ions. This is the fastest DNAzyme reported for this reaction, and it might enable applications in chemical biology. As a proof‐of‐concept, using this DNAzyme, the reactions between phosphorothioate‐modified RNA and strongly thiophilic metals (Hg 2+ and Tl 3+ ) were studied as a function of pH.