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Use of Methylmalonyl‐CoA Epimerase in Enhancing Crotonase Stereoselectivity
Author(s) -
Hamed Refaat B.,
GomezCastellanos J. Ruben,
Sean Froese D.,
Krysztofinska Ewelina,
Yue Wyatt W.,
Schofield Christopher J.
Publication year - 2016
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201500644
Subject(s) - stereoselectivity , biocatalysis , chemistry , pyruvate carboxylase , reductase , enzyme , stereochemistry , biochemistry , catalysis , reaction mechanism
The use of methylmalonyl‐CoA epimerase (MCEE) to improve stereoselectivity in crotonase‐mediated biocatalysis is exemplified by the coupling of MCEE, crotonyl‐CoA carboxylase reductase and carboxymethylproline synthase in a three‐enzyme one‐pot sequential synthesis of functionalised C‐5 carboxyalkylprolines starting from crotonyl‐CoA and carbon dioxide.