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Development of Fluorescent Protein Probes Specific for Parallel DNA and RNA G‐Quadruplexes
Author(s) -
Dang Dung Thanh,
Phan Anh Tuân
Publication year - 2016
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201500503
Subject(s) - fluorescence , g quadruplex , rna , dna , green fluorescent protein , chemistry , helicase , biophysics , biochemistry , microbiology and biotechnology , biology , gene , physics , quantum mechanics
We have developed fluorescent protein probes specific for parallel G‐quadruplexes by attaching cyan fluorescent protein to the G‐quadruplex‐binding motif of the RNA helicase RHAU. Fluorescent probes containing RHAU peptide fragments of different lengths were constructed, and their binding to G‐quadruplexes was characterized. The selective recognition and discrimination of G‐quadruplex topologies by the fluorescent protein probes was easily detected by the naked eye or by conventional gel imaging.
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