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Inside Back Cover: Artifact‐Free Quantification and Sequencing of Rare Recombinant Viruses by Using Drop‐Based Microfluidics (ChemBioChem 15/2015)
Author(s) -
Tao Ye,
Rotem Assaf,
Zhang Huidan,
Cockrell Shelley K.,
Koehler Stephan A.,
Chang Connie B.,
Ung Lloyd W.,
Cantalupo Paul G.,
Ren Yukun,
Lin Jeffrey S.,
Feldman Andrew B.,
Wobus Christiane E.,
Pipas James M.,
Weitz David A.
Publication year - 2015
Publication title -
chembiochem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201500460
Subject(s) - microfluidics , biology , computational biology , genome , synthetic biology , dna sequencing , nanotechnology , genetics , dna , materials science , gene
The inside back cover picture shows a novel drop‐based microfluidics method to detect very rare viral recombinants. Viral recombination has a significant impact on virus evolution and epidemiology; however, a number of challenges prevent its accurate quantification and mechanism analysis, including low frequency, the uncertainty of breakpoints and chimeric artifacts generated during amplification due to the co‐existing different parent viruses. The core advantage of our method originates in compartmentalization of single viral genomes; this fundamentally avoids the generation of chimeras. Furthermore, the high‐throughput isolation of the recombinants by using drop‐based fluorescence sorting allows the screening of ∼10 7 viral genomes one by one in two hours.