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Pre‐organized Guide RNA in the Cas9 Complex Is Ready for the Selection of Target Double‐Stranded DNA
Author(s) -
Kamiya Yukiko,
Asanuma Hiroyuki
Publication year - 2015
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201500424
Subject(s) - cas9 , dna , subgenomic mrna , guide rna , rna , crispr , computational biology , chemistry , biology , biophysics , genetics , gene
The 3 D structure of Cas9 bound with sgRNA was solved by X‐ray crystal‐structure analysis. The conformation change in SpyCas9 upon binding to sgRNA changes Cas9 to target‐DNA‐recognition mode in which seed segments in sgRNA are pre‐ordered in an A‐type conformation.