Premium
Directed Evolution of Enzymes for Industrial Biocatalysis
Author(s) -
Porter Joanne L.,
Rusli Rukhairul A.,
Ollis David L.
Publication year - 2016
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201500280
Subject(s) - directed evolution , biochemical engineering , biocatalysis , environmentally friendly , protein engineering , synthetic biology , directed molecular evolution , bioremediation , pharmaceutics , microbiology and biotechnology , computer science , nanotechnology , enzyme , computational biology , engineering , chemistry , biology , biochemistry , materials science , catalysis , ecology , ionic liquid , genetics , bacteria , mutant , pharmacology , gene
Enzymes have the potential to catalyse a wide variety of chemical reactions. They are increasingly being sought as environmentally friendly and cost‐effective alternatives to conventional catalysts used in industries ranging from bioremediation to applications in medicine and pharmaceutics. Despite the benefits, they are not without their limitations. Many naturally occurring enzymes are not suitable for use outside of their native cellular environments. However, protein engineering can be used to generate enzymes tailored for specific industrial applications. Directed evolution is particularly useful and can be employed even when lack of structural information impedes the use of rational design. The aim of this review is to provide an overview of current industrial applications of enzyme technology and to show how directed evolution can be used to modify and to enhance enzyme properties. This includes a brief discussion on library generation and a more detailed focus on library screening methods, which are critical to any directed evolution experiment.