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Protein Synthesis in Sub‐Micrometer Water‐in‐Oil Droplets
Author(s) -
Gallo Valentina,
Stano Pasquale,
Luisi Pier Luigi
Publication year - 2015
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201500274
Subject(s) - lecithin , chemistry , micrometer , green fluorescent protein , chemical engineering , biophysics , biosynthesis , nanotechnology , materials science , biochemistry , biology , enzyme , physics , optics , gene , engineering
Water‐in‐oil (w/o) emulsions are used as a cellular model because of their unique cell‐like architecture. Previous works showed the capability of eukaryotic‐cell‐sized w/o droplets (5–50 μm) to support protein synthesis efficiently; however data about smaller w/o compartments (<1 μm) are lacking. This work focuses on the biosynthesis of the enhanced green fluorescent protein (EGFP) inside sub‐micrometric lecithin‐based w/o droplets (0.8–1 μm) and on its dependence on the compartments’ dynamic properties in terms of solute exchange mechanisms. We demonstrated that protein synthesis is strongly affected by the nature of the lipid interface. These findings could be of value and interest for both basic and applied research.