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Folic‐Acid‐Targeted Self‐Assembling Supramolecular Carrier for Gene Delivery
Author(s) -
Liao Rongqiang,
Yi Shouhui,
Liu Manshuo,
Jin Wenling,
Yang Bo
Publication year - 2015
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201500208
Subject(s) - polyethylenimine , adamantane , ethylene glycol , peg ratio , chemistry , gene delivery , transfection , nucleic acid , cytotoxicity , drug carrier , combinatorial chemistry , biophysics , drug delivery , biochemistry , organic chemistry , gene , in vitro , biology , finance , economics
A targeting gene carrier for cancer‐specific delivery was successfully developed through a “multilayer bricks‐mortar” strategy. The gene carrier was composed of adamantane‐functionalized folic acid (FA‐AD), an adamantane‐functionalized poly(ethylene glycol) derivative (PEG‐AD), and β‐cyclodextrin‐grafted low‐molecular‐weight branched polyethylenimine (PEI‐CD). Carriers produced by two different self‐assembly schemes, involving either precomplexation of the PEI‐CD with the FA‐AD and PEG‐AD before pDNA condensation (Method A) or pDNA condensation with the PEI‐CD prior to addition of the FA‐AD and PEG‐AD to engage host–guest complexation (Method B) were investigated for their ability to compact pDNA into nanoparticles. Cell viability studies show that the material produced by the Method A assembly scheme has lower cytotoxicity than branched PEI 25 kDa (PEI‐25KD) and that the transfection efficiency is maintained. These findings suggest that the gene carrier, based on multivalent host–guest interactions, could be an effective, targeted, and low‐toxicity carrier for delivering nucleic acid to target cells.