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Detection of Human α‐ L ‐Fucosidases by a Quinone Methide‐Generating Probe: Enhanced Activities in Response to Helicobacter pylori Infection
Author(s) -
Nandakumar Manjula,
Hsu YuLing,
Lin Jason C.Y,
Lo Chun,
Lo LeeChiang,
Lin ChunHung
Publication year - 2015
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201500178
Subject(s) - helicobacter pylori , context (archaeology) , intracellular , in vivo , quinone methide , chemistry , enzyme , microbiology and biotechnology , biochemistry , biology , quinone , paleontology , genetics
α‐ L ‐Fucosidase activity is associated with several diseases. To study the enzymatic activity change under pathological conditions, we developed a quinone methide‐generating activity‐based probe useful for examining the presence, activity, and localization of human α‐ L ‐fucosidase in vivo in the context of Helicobacter pylori infection. In particular, an increase in intracellular fucosidase (Fuca1) activity was found in gastric epithelial cells upon bacterial infection. We further studied the effect of several bacterial stimulants on this enhanced Fuca1 activity and identified lipopolysaccharides to be a major contributing factor.