Premium
Deoxycholate‐Based Glycosides (DCGs) for Membrane Protein Stabilisation
Author(s) -
Bae Hyoung Eun,
Gotfryd Kamil,
Thomas Jennifer,
Hussain Hazrat,
Ehsan Muhammad,
Go Juyeon,
Loland Claus J.,
Byrne Bernadette,
Chae Pil Seok
Publication year - 2015
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201500151
Subject(s) - amphiphile , membrane , membrane protein , chemistry , biochemistry , biophysics , biology , organic chemistry , copolymer , polymer
Detergents are an absolute requirement for studying the structure of membrane proteins. However, many conventional detergents fail to stabilise denaturation‐sensitive membrane proteins, such as eukaryotic proteins and membrane protein complexes. New amphipathic agents with enhanced efficacy in stabilising membrane proteins will be helpful in overcoming the barriers to studying membrane protein structures. We have prepared a number of deoxycholate‐based amphiphiles with carbohydrate head groups, designated deoxycholate‐based glycosides (DCGs). These DCGs are the hydrophilic variants of previously reported deoxycholate‐based N‐oxides (DCAOs). Membrane proteins in these agents, particularly the branched diglucoside‐bearing amphiphiles DCG‐1 and DCG‐2, displayed favourable behaviour compared to previously reported parent compounds (DCAOs) and conventional detergents (LDAO and DDM). Given their excellent properties, these agents should have significant potential for membrane protein studies.