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Biosynthesis of Sesterterpenes, Head‐to‐Tail Triterpenes, and Sesquarterpenes in Bacillus clausii : Identification of Multifunctional Enzymes and Analysis of Isoprenoid Metabolites
Author(s) -
Ueda Daijiro,
Yamaga Hiroaki,
Murakami Mizuki,
Totsuka Yusuke,
Shinada Tetsuro,
Sato Tsutomu
Publication year - 2015
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201500138
Subject(s) - terpene , terpenoid , biosynthesis , enzyme , atp synthase , biochemistry , stereochemistry , chemistry , biology
We performed functional analysis of recombinant enzymes and analysis of isoprenoid metabolites in Bacillus clausii to gain insights into the biosynthesis of rare terpenoid groups of sesterterpenes, head‐to‐tail triterpenes, and sesquarterpenes. We have identified an (all‐ E )‐isoprenyl diphosphate synthase (E‐IDS) homologue as a trifunctional geranylfarnesyl diphosphate (GFPP)/hexaprenyl diphosphate (HexPP)/heptaprenyl diphosphate (HepPP) synthase. In addition, we have redefined the function of a tetraprenyl‐β‐curcumene synthase homologue as that of a trifunctional sesterterpene/triterpene/sesquarterpene synthase. This study has revealed that GFPP, HexPP, and HepPP, intermediates of two isoprenoid pathways (acyclic terpenes and menaquinones), are biosynthesized by one trifunctional E‐IDS. In addition, GFPP/HexPP and HepPP are the primary substrates for the biosynthesis of acyclic terpenes and menaquinone‐7, respectively.