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Simple “On‐Demand” Production of Bioactive Natural Products
Author(s) -
Bode Edna,
Brachmann Alexander O.,
Kegler Carsten,
Simsek Rukayye,
Dauth Christina,
Zhou Qiuqin,
Kaiser Marcel,
Klemmt Petra,
Bode Helge B.
Publication year - 2015
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201500094
Subject(s) - natural product , biosynthesis , gene , bacteria , biochemistry , biology , chemistry , genetics
Exchange of the native promoter to the arabinose‐inducible promoter P BAD was established in entomopathogenic bacteria to silence and/or activate gene clusters involved in natural product biosynthesis. This allowed the “on‐demand” production of GameXPeptides, xenoamicins, and the blue pigment indigoidine. The gene clusters for the novel “mevalagmapeptides” and the highly toxic xenorhabdins were identified by this approach.