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Engineering a Bacterial Tape Recorder
Author(s) -
Prokup Alexander,
Deiters Alexander
Publication year - 2015
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201500061
Subject(s) - toolbox , modular design , computer science , scalability , synthetic biology , dna , reading (process) , function (biology) , computational biology , recombinase , computer hardware , biology , genetics , programming language , database , political science , recombination , law , gene
A method has been developed to produce and integrate single‐stranded DNA into genomic locations in bacteria in response to exogenous signals. The system functions similarly to a cellular tape recorder by writing information into DNA and reading it at a later time. Much like other cellular memory platforms, its operation is based on DNA recombinase function. However, the scalability and recording capacity have been improved over previous designs. In addition, memory storage was reversible and could be recorded in response to analogue inputs, such as light exposure. This modular memory writing system is an important addition to the genomic editing toolbox available for synthetic biology.