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Formation and Abundance of 5‐Hydroxymethylcytosine in RNA
Author(s) -
Huber Sabrina M.,
van Delft Pieter,
Mendil Lee,
Bachman Martin,
Smollett Katherine,
Werner Finn,
Miska Eric A.,
Balasubramanian Shankar
Publication year - 2015
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201500013
Subject(s) - rna , transcription (linguistics) , biology , rna methylation , ribonucleoside , messenger rna , methylation , microbiology and biotechnology , 5 hydroxymethylcytosine , chemistry , non coding rna , gene , biochemistry , gene expression , dna methylation , methyltransferase , philosophy , linguistics
RNA methylation is emerging as a regulatory RNA modification that could have important roles in the control and coordination of gene transcription and protein translation. Herein, we describe an in vivo isotope‐tracing methodology to demonstrate that the ribonucleoside 5‐methylcytidine (m 5 C) is subject to oxidative processing in mammals, forming 5‐hydroxymethylcytidine (hm 5 C) and 5‐formylcytidine (f 5 C). Furthermore, we have identified hm 5 C in total RNA from all three domains of life and in polyA‐enriched RNA fractions from mammalian cells. This suggests m 5 C oxidation is a conserved process that could have critical regulatory functions inside cells.