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Cover Picture: Comparison of the Reactivity of Carbohydrate Photoaffinity Probes with Different Photoreactive Groups (ChemBioChem 10/2014)
Author(s) -
Sakurai Kaori,
Ozawa Shimpei,
Yamada Rika,
Yasui Tomoki,
Mizuno Sakae
Publication year - 2014
Publication title -
chembiochem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201490032
Subject(s) - diazirine , photoaffinity labeling , chemistry , carbohydrate chemistry , azide , bioorthogonal chemistry , click chemistry , combinatorial chemistry , stereochemistry , organic chemistry , binding site , biochemistry
The cover picture shows an in‐gel fluorescence imaging analysis comparing the photoaffinity labeling reaction between peanut agglutinin (PNA) in HeLa cell lysate and carbohydrate photoaffinity probes bearing three major photoreactive groups: benzophenone, aryl azide, and alkyl diazirine. Only the diazirine probe achieved highly selective crosslinking of PNA, which represented a low‐affinity binding protein. An image of a bound complex between the diazirine‐bearing carbohydrate photoaffinity probe (in ball and stick representation) and PNA (in ribbon representation) is also shown. For details, see the paper by K. Sakurai et al. on p. 1399 ff.