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Insect‐Specific Production of New GameXPeptides in Photorhabdus luminescens TTO1, Widespread Natural Products in Entomopathogenic Bacteria
Author(s) -
Nollmann Friederike I.,
Dauth Christina,
Mulley Geraldine,
Kegler Carsten,
Kaiser Marcel,
Waterfield Nick R.,
Bode Helge B.
Publication year - 2015
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201402603
Subject(s) - photorhabdus luminescens , photorhabdus , biology , heterologous , heterologous expression , xenorhabdus , bacteria , microbiology and biotechnology , gene , recombinant dna , genetics
Discovery of new natural products by heterologous expression reaches its limits, especially when specific building blocks are missing in the heterologous host or the production medium. Here, we describe the insect‐specific production of the new GameXPeptides E–H ( 5 – 8 ) from Photorhabdus luminescens TTO1, which can be produced heterologously from expression of the GameXPeptide synthetase GxpS only upon supplementation of the production media with the missing building blocks, and thus must be regarded as the true natural products under natural conditions.