Functional Characterization of MpaG′, the O‐Methyltransferase Involved in the Biosynthesis of Mycophenolic Acid | Zendy

Zhang Wei | Zendy; Cao Shaona | Zendy; Qiu Li | Zendy; Qi Fengxia | Zendy; Li Zhong | Zendy; Yang Ying | Zendy; Huang Shaohua | Zendy; Bai Fali | Zendy; Liu Changning | Zendy; Wan Xiaobo | Zendy; Li Shengying | Zendy

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Functional Characterization of MpaG′, the O‐Methyltransferase Involved in the Biosynthesis of Mycophenolic Acid

Author(s) -

Zhang Wei,

Cao Shaona,

Qiu Li,

Qi Fengxia,

Li Zhong,

Yang Ying,

Huang Shaohua,

Bai Fali,

Liu Changning,

Wan Xiaobo,

Li Shengying

Publication year - 2015

Publication title -

chembiochem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.05

H-Index - 126

eISSN - 1439-7633

pISSN - 1439-4227

DOI - 10.1002/cbic.201402600

Subject(s) - methyltransferase , mycophenolic acid , biosynthesis , methylation , o methyltransferase , gene cluster , chemistry , biochemistry , methionine , gene , in vitro , mycophenolate , substrate (aquarium) , stereochemistry , biology , amino acid , medicine , transplantation , surgery , ecology

Mycophenolic acid (MPA, 1 ) is a clinically important immunosuppressant. In this report, a gene cluster mpa′ responsible for the biosynthesis of 1 was identified from Penicillium brevicompactum NRRL 864. The S ‐adenosyl‐ L ‐methionine‐dependent (SAM‐dependent) O‐methyltransferase encoded by the mpaG′ gene was functionally and kinetically characterized in vitro. MpaG′ catalyzes the methylation of demethylmycophenolic acid (DMMPA, 6 ) to form 1 . It also showed significant substrate flexibility by methylating two structural derivatives of 6 prepared by organic synthesis.

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