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Elucidating pH‐Dependent Collagen Triple Helix Formation through Interstrand Hydroxyproline‐Glutamic Acid Interactions
Author(s) -
Chen Liwei,
Cai Shuting,
Lim Jaehong,
Lee Su Seong,
Lee SongGil
Publication year - 2015
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201402570
Subject(s) - triple helix , collagen helix , glutamic acid , hydroxyproline , chemistry , helix (gastropod) , amino acid , stereochemistry , biochemistry , biology , ecology , snail
Here, we describe systematic explorations into the molecular basis underlying hydroxyproline‐mediated interstrand interactions on the triple‐helical stability of collagen‐mimetic peptides containing glutamic acid residues. Our studies reveal that the triple‐helical stability of these peptides relies on the existence of interstrand interactions between hydroxyprolines and glutamic acid residues that are pH dependent. These unique interactions have been used to engineer collagen peptides that form triple helices on demand through pH control.