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Mixed Carbonates as Useful Substrates for a Fluorogenic Assay for Lipases and Esterases
Author(s) -
Zadlo Anna,
Koszelewski Dominik,
Borys Filip,
Ostaszewski Ryszard
Publication year - 2015
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201402528
Subject(s) - esterase , lipase , chemistry , high throughput screening , hydrolysis , enzyme , combinatorial chemistry , biocatalysis , chromatography , biochemistry , catalysis , reaction mechanism
Mixed carbonates of 7‐hydroxy‐4‐methylcoumarin were shown to be a new class of probe for fluorogenic assays. They are promising substrates for fingerprinting enzyme hydrolytic activity, and proved particularly useful because of the low level of nonspecific degradation and ease of synthesis. They are highly relevant for screening lipase and esterase libraries. These advantages make umbelliferyl carbonates highly suitable substrates for high‐throughput screening. Moreover, we report the use of chiral fluorogenic carbonates as enantiopreference probes.