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CHoMP: A Chemoenzymatic Histology Method Using Clickable Probes
Author(s) -
Rouhanifard Sara H.,
LópezAguilar Aimé,
Wu Peng
Publication year - 2014
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201402433
Subject(s) - glycosylation , bioorthogonal chemistry , glycan , muc1 , glycosyltransferase , click chemistry , chemistry , histology , mucin , biochemistry , computational biology , glycoprotein , pathology , biology , combinatorial chemistry , medicine , gene
The characterization of aberrant glycosylation patterns in biopsied patient samples represents a remarkable challenge for scientists and medical doctors due to the lack of specific methods for detection. Here, we report the development of a histological method, dubbed CHoMP—chemoenzymatic histology of membrane polysaccharides—for analyzing glycosylation patterns in mammalian tissues. This method exploits a recombinant glycosyltransferase to transfer a monosaccharide analogue equipped with a chemical handle to a specific cell‐surface glycan target, which can then be derivatized with imaging probes by using bioorthogonal click chemistry for visualization. We applied CHoMP to survey changes in expression of N ‐acetyllactosamine (LacNAc) in human samples from patients afflicted with lung adenocarcinoma and observed a sharp decrease in expression levels between normal and early grade tumors, thus suggesting a potential application of this technique in early cancer diagnosis.