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Involvement of Ralfuranone Production in the Virulence of Ralstonia solanacearum OE1‐1
Author(s) -
Kai Kenji,
Ohnishi Hideyuki,
Mori Yuka,
Kiba Akinori,
Ohnishi Kouhei,
Hikichi Yasufumi
Publication year - 2014
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201402404
Subject(s) - ralstonia solanacearum , virulence , complementation , bacterial wilt , biology , quorum sensing , microbiology and biotechnology , gene , ralstonia , strain (injury) , genetics , phenotype , pathogen , anatomy
Ralstonia solanacearum causes a destructive disease called “bacterial wilt” in numerous plant species. Its virulence is controlled by the transcriptional regulator PhcA, the activity of which is, in turn, regulated in a cell‐density dependent manner, termed quorum sensing. We herein described the identification and characterization of ralfuranones J–L, new PhcA‐regulated secondary metabolites, and the known derivatives, ralfuranones A and B, from R. solanacearum strain OE1‐1. Their structures were determined by spectroscopic and chemical methods. These ralfuranones were also detected in vascular exudates from host plants infected with OE1‐1. Deletion of ralA , which encodes an enzyme for ralfuranone biosynthesis, reduced the virulence of OE1‐1 in tomato plants. Virulence was restored by complementation of the ralA gene. The results suggest that ralfuranones play important roles in the virulence of OE1‐1.