Premium
High‐Affinity Carbohydrate Binding by Trimeric Benzoboroxoles Measured on Carbohydrate Arrays
Author(s) -
Claes Dorith,
Memmel Elisabeth,
Holzapfel Malte,
Seibel Jürgen,
Maison Wolfgang
Publication year - 2014
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201402297
Subject(s) - binding affinities , affinities , fluorescence , carbohydrate , chemistry , lectin , fluorescence spectroscopy , biochemistry , biophysics , stereochemistry , biology , receptor , physics , quantum mechanics
Carbohydrates are involved in a wide range of biological processes of pharmaceutical relevance. The selective recognition of carbohydrates is therefore of great interest in biology and medicine. In this study we present the synthesis of fluorescent multimeric benzoboroxoles and the analysis of multivalent binding processes to immobilized carbohydrate arrays by fluorescence spectroscopy. We observed high binding affinities of trimeric benzoboroxoles by determination of K Dsurf values for their interaction with α‐Gal on glass chips. The observed K Dsurf values were in the mid‐n M range (49 and 104 n M ) and are comparable to the K Dsurf values for binding of natural lectins, such as that of ConA to immobilized α‐Man (79 n M ). The array technology was found to be an excellent tool for studying the binding processes of multivalent lectin mimetics with respect to profiling and quantitation.