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Tissue‐Specific Proteome Tagging: An Orthogonal Approach
Author(s) -
Snyder Adrienne L.,
Brustad Eric M.
Publication year - 2014
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201402210
Subject(s) - proteome , cyclopropene , amino acid , transfer rna , computational biology , biochemistry , biology , chemistry , aminoacyl trna synthetase , cell sorting , genetic code , cell , gene , rna , medicinal chemistry
SORTing through the proteome: A versatile method has been developed for the incorporation of a novel 1,3‐disubstituted cyclopropene amino acid into the proteome in response to a variety of sense codons and subsequent labeling. Incorporation can be confined to certain tissues by the selective expression of an orthogonal tRNA synthetase under cell‐selective promoters.