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Characterization of the Calicheamicin Orsellinate C2‐ O ‐Methyltransferase CalO6
Author(s) -
Singh Shanteri,
Nandurkar Nitin S.,
Thorson Jon S.
Publication year - 2014
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201402119
Subject(s) - biosynthesis , calicheamicin , hydroxylation , polyketide , methyltransferase , biochemistry , methylation , chemistry , halogenation , enzyme , stereochemistry , biology , dna , genetics , organic chemistry , antibody
Although bacterial iterative type I polyketide synthases are now known to participate in the biosynthesis of a small set of diverse natural products, the subsequent downstream modification of the resulting polyketide products is poorly understood. We report the functional characterization of the putative orsellinic acid C2‐ O ‐methyltransferase, which is involved in calicheamicin biosynthesis. This study suggests that C2‐O‐methylation precedes C3‐hydroxylation/methylation and C5‐iodination and requires a coenzyme A‐ or acyl carrier protein‐bound substrate.