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Using Chemistry to Investigate the Molecular Consequences of Protein Ubiquitylation
Author(s) -
Abeywardana Tharindumala,
Pratt Matthew R.
Publication year - 2014
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201402117
Subject(s) - chemistry , ubiquitin , protein chemistry , chemical biology , homogeneous , posttranslational modification , chemical synthesis , biochemistry , nanotechnology , computational biology , biology , enzyme , in vitro , materials science , physics , gene , thermodynamics
Abstract Chemistry has long played an indispensable role in biological discovery through the synthesis of homogeneous, structurally defined material. With continuing advances in the area of synthetic protein chemistry, chemists are able to prepare increasingly large and complex proteins that have enabled key biochemical experiments. Here, we describe some of the chemical methods that have been applied to the synthesis of ubiquitylated proteins, as ubiquitylation is a crucial post‐translational modification that mediates a variety of important biological effects on substrate proteins.