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DNA‐Scaffolded Multivalent Ligands to Modulate Cell Function
Author(s) -
Zhang Zhiqing,
Eckert Mark A.,
Ali M. Monsur,
Liu Linan,
Kang DongKu,
Chang Elizabeth,
Pone Egest J.,
Sender Leonard S.,
Fruman David A.,
Zhao Weian
Publication year - 2014
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201402100
Subject(s) - ligand (biochemistry) , dna , function (biology) , receptor , chemistry , cell , cancer cell , microbiology and biotechnology , apoptosis , signal transduction , computational biology , biophysics , biology , cancer , biochemistry , genetics
We report a simple, versatile, multivalent ligand system that is capable of specifically and efficiently modulating cell‐surface receptor clustering and function. The multivalent ligand is made of a polymeric DNA scaffold decorated with biorecognition ligands (i.e., antibodies) to interrogate and modulate cell receptor signaling and function. Using CD20 clustering‐mediated apoptosis in B‐cell cancer cells as a model system, we demonstrated that our multivalent ligand is significantly more effective at inducing apoptosis of target cancer cells than its monovalent counterpart. This multivalent DNA material approach represents a new chemical biology tool to interrogate cell receptor signaling and functions and to potentially manipulate such functions for the development of therapeutics.