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Microarray‐Based Identification of Lectins for the Purification of Human Urinary Extracellular Vesicles Directly from Urine Samples
Author(s) -
Echevarria Juan,
Royo Felix,
Pazos Raquel,
Salazar Lorena,
FalconPerez Juan Manuel,
Reichardt NielsChristian
Publication year - 2014
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201402058
Subject(s) - extracellular vesicles , urine , chemistry , urinary system , extracellular , identification (biology) , biological fluids , lectin , biochemistry , chromatography , biology , microbiology and biotechnology , botany , endocrinology
As cellular‐derived vesicles largely maintain the biomolecule composition of their original tissue, exosomes, which are found in nearly all body fluids, have enormous potential as clinical disease markers. A major bottleneck in the development of exosome‐based diagnostic assays is the challenging purification of these vesicles; this requires time‐consuming and instrument‐based procedures. We employed lectin arrays to identify potential lectins as probes for affinity‐based isolation of exosomes from the urinary matrix. We found three lectins that showed specific interactions to vesicles and no (or only residual) interaction with matrix proteins. Based on these findings a bead‐based method for lectin‐based isolation of exosomes from urine was developed as a sample preparation step for exosome‐based biomarker research.