Selective DNA‐Binding by Designed Bisbenzamidine‐Homeodomain Chimeras | Zendy

Mosquera Jesús | Zendy; Rodríguez Jéssica | Zendy; Vázquez M. Eugenio | Zendy; Mascareñas José L. | Zendy

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Selective DNA‐Binding by Designed Bisbenzamidine‐Homeodomain Chimeras

Author(s) -

Mosquera Jesús,

Rodríguez Jéssica,

Vázquez M. Eugenio,

Mascareñas José L.

Publication year - 2014

Publication title -

chembiochem

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.05

H-Index - 126

eISSN - 1439-7633

pISSN - 1439-4227

DOI - 10.1002/cbic.201400079

Subject(s) - engrailed , homeobox , minor groove , dna , helix (gastropod) , biology , biophysics , chemistry , genetics , microbiology and biotechnology , gene , transcription factor , ecology , snail

We report the construction of conjugates between three variants of the helix 3 region of a Q50K engrailed homeodomain and bisbenzamidine minor‐groove DNA binders. The hybrid featuring the sequence of the native protein failed to bind to DNA; however, modifications that increased the α‐helical folding propensity of the peptide allowed specific DNA binding by a bipartite (major/minor groove) interaction.

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