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Selective DNA‐Binding by Designed Bisbenzamidine‐Homeodomain Chimeras
Author(s) -
Mosquera Jesús,
Rodríguez Jéssica,
Vázquez M. Eugenio,
Mascareñas José L.
Publication year - 2014
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201400079
Subject(s) - engrailed , homeobox , minor groove , dna , helix (gastropod) , biology , biophysics , chemistry , genetics , microbiology and biotechnology , gene , transcription factor , ecology , snail
We report the construction of conjugates between three variants of the helix 3 region of a Q50K engrailed homeodomain and bisbenzamidine minor‐groove DNA binders. The hybrid featuring the sequence of the native protein failed to bind to DNA; however, modifications that increased the α‐helical folding propensity of the peptide allowed specific DNA binding by a bipartite (major/minor groove) interaction.