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Inside Cover: Highly Efficient Förster Resonance Energy Transfer in a Fast, Serum‐Compatible Immunoassay (ChemBioChem 6/2013)
Author(s) -
Kreisig Thomas,
Hoffmann Ralf,
Zuchner Thole
Publication year - 2013
Publication title -
chembiochem
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201390017
Subject(s) - förster resonance energy transfer , immunoassay , homogeneous , cover (algebra) , chemistry , linear range , energy transfer , nanotechnology , detection limit , chromatography , physics , materials science , fluorescence , optics , antibody , chemical physics , biology , immunology , mechanical engineering , engineering , thermodynamics
The inside cover picture shows the assay principle of a FRET‐based homogeneous immunoassay described by T. Zuchner et al. on p. 699 ff. When using the novel phosphorescent dye EuLH and BHQ‐10 as the FRET pair, the FRET efficiency in this homogeneous, microplate‐based immunoassay increases to >99.5 %, thus leading to significantly improved signal‐to‐background ratio, precision and linear range.