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An Improved Technique for the Rapid Chemical Characterisation of Bacterial Terpene Cyclases
Author(s) -
Dickschat Jeroen S.,
Pahirulzaman Khomaizon A. K.,
Rabe Patrick,
Klapschinski Tim A.
Publication year - 2014
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201300763
Subject(s) - ura3 , yeast , heterologous expression , biology , cloning (programming) , heterologous , selectable marker , expression vector , plasmid , gene , vector (molecular biology) , computational biology , terpene , biochemistry , saccharomyces cerevisiae , genetics , recombinant dna , computer science , programming language
A derivative of the pET28c(+) expression vector was constructed. It contains a yeast replication system (2μ origin of replication) and a yeast selectable marker (URA3), and can be used for gene cloning in yeast by efficient homologous recombination, and for heterologous expression in E. coli . The vector was used for the expression and chemical characterisation of three bacterial terpene cyclases.