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Structure Elucidation of DNA–Protein Crosslinks by Using Reductive Desulfurization and Liquid Chromatography–Tandem Mass Spectrometry
Author(s) -
Wickramaratne Susith,
Tretyakova Natalia Y.
Publication year - 2014
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201300757
Subject(s) - thioether , chemistry , mass spectrometry , adduct , dna , flue gas desulfurization , tandem mass spectrometry , liquid chromatography–mass spectrometry , combinatorial chemistry , cysteine , chromatography , organic chemistry , biochemistry , enzyme
Easier with ethyl: Guengerich and co‐workers have developed a powerful new approach to the structure elucidation of hydrolytically stable AGT–DNA crosslinks by reductive desulfurization of the thioether linkage between AGT and DNA to convert cysteine DPCs to the corresponding ethyl–DNA adducts, which can be readily characterized by LC‐MS n .