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The Efficacies of Cell‐Penetrating Peptides in Accumulating in Large Unilamellar Vesicles Depend on their Ability To Form Inverted Micelles
Author(s) -
Swiecicki JeanMarie,
Bartsch Annika,
Tailhades Julien,
Di Pisa Margherita,
Heller Benjamin,
Chassaing Gérard,
Mansuy Christelle,
Burlina Fabienne,
Lavielle Solange
Publication year - 2014
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201300742
Subject(s) - vesicle , chemistry , micelle , phospholipid , biophysics , bilayer , lipid bilayer , peptide , cell penetrating peptide , chromosomal translocation , ternary operation , membrane , chromatography , biochemistry , organic chemistry , aqueous solution , biology , gene , computer science , programming language
Abstract In this study, the direct translocation of cell‐penetrating peptides (CPPs) into large unilamellar vesicles (LUVs) was shown to be rapid for all the most commonly used CPPs. This translocation led within a few minutes to intravesicular accumulation up to 0.5 m M , with no need for a transbilayer potential. The accumulation of CPPs inside LUVs was found to depend on CPP sequence, CPP extravesicular concentration and phospholipid (PL) composition, either in binary or ternary mixtures of PLs. More interestingly, the role of anionic phospholipid flip‐flopping in the translocation process was ascertained. CPPs enhanced the flipping of PLs, and the intravesicular CPP accumulation directly correlated with the amount of anionic PLs that had been transferred from the external to the internal leaflet of the LUV bilayer, thus demonstrating the transport of peptide/lipid complexes as inverted micelles.