Premium
Identification and Biosynthesis of New Acyloins from the Thermophilic Bacterium Thermosporothrix hazakensis SK20 ‐ 1 T
Author(s) -
Park JinSoo,
Kagaya Noritaka,
Hashimoto Junko,
Izumikawa Miho,
Yabe Shuhei,
Shinya Kazuo,
Nishiyama Makoto,
Kuzuyama Tomohisa
Publication year - 2014
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201300690
Subject(s) - thermophile , bacteria , biosynthesis , enzyme , chemistry , thiamine , biochemistry , catalysis , stereochemistry , combinatorial chemistry , biology , genetics
Two new acyloin compounds were isolated from the thermophilic bacterium Thermosporothrix hazakensis SK20‐1 T . Genome sequencing of the bacterium and biochemical studies identified the thiamine diphosphate (TPP)‐dependent enzyme Thzk0150, which is involved in the formation of acyloin. Through extensive analysis of the Thzk0150‐catalyzed reaction products, we propose a putative reaction mechanism involving two substrates: 4‐methyl‐2‐oxovalerate as an acyl donor and phenyl pyruvate as an acyl acceptor.