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A Light‐Activated Metal Complex Targets both DNA and RNA in a Fluorescent in Vitro Transcription and Translation Assay
Author(s) -
Heidary David K.,
Glazer Edith C.
Publication year - 2014
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201300681
Subject(s) - dna , in vitro , transcription (linguistics) , cytotoxicity , rna , fluorescence , green fluorescent protein , microbiology and biotechnology , chemistry , biology , potency , transcription factor , messenger rna , biophysics , biochemistry , gene , philosophy , linguistics , physics , quantum mechanics
A coupled in vitro transcription and translation (IVTT) assay that uses GFP as a fluorescent reporter allowed the potency of a light‐activated cytotoxic ruthenium agent to be quantified. The compound inhibits the function of both DNA and mRNA only upon light activation. The IVTT functional assay provides estimates of potency that are consistent with cellular cytotoxicity values, in marked contrast to the values obtained from traditional DNA‐damage assays.