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Rapid Determination of the Amino Acid Configuration of Xenotetrapeptide
Author(s) -
Kegler Carsten,
Nollmann Friederike I.,
Ahrendt Tilman,
Fleischhacker Florian,
Bode Edna,
Bode Helge B.
Publication year - 2014
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201300602
Subject(s) - amino acid , peptide , strain (injury) , chemistry , mass spectrometry , amino acid analysis , biochemistry , stereochemistry , biology , chromatography , anatomy
An E. coli strain with deletions in five transaminases (Δ aspC Δ ilvE Δ tyrB Δ avtA Δ ybfQ ) was constructed to be unable to degrade several amino acids. This strain was used as an expression host for the analysis of the amino acid configuration of nonribosomally synthesized peptides, including the novel peptide “xenotetrapeptide” from Xenorhabdus nematophila , by using a combination of labeling experiments and mass spectrometry. Additionally, the number of D ‐amino acids in the produced peptide was assigned following simple cultivation of the expression strain in D 2 O.