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Biosynthesis of Hygrocins, Antitumor Naphthoquinone Ansamycins Produced by Streptomyces sp. LZ35
Author(s) -
Li Shanren,
Wang Haoxin,
Li Yaoyao,
Deng Jingjing,
Lu Chunhua,
Shen Yan,
Shen Yuemao
Publication year - 2014
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201300599
Subject(s) - naphthoquinone , monooxygenase , biosynthesis , gene cluster , streptomyces , mutant , gene , biochemistry , enzyme , biology , chemistry , genetics , cytochrome p450 , bacteria , botany
Abstract Hygrocins are naphthoquinone ansamycins with significant antitumor activities. Here, we report the identification and characterization of the hygrocin biosynthetic gene cluster (hgc) in Streptomyces sp. LZ35. A biosynthetic pathway is proposed based on bioinformatics analysis of the hgc genes and intermediates accumulated in selected gene disruption mutants. One of the steps during the biosynthesis of hygrocins is a Baeyer–Villiger oxidation between C5 and C6, catalyzed by luciferase‐like monooxygenase homologue Hgc3. Hgc3 represents the founding member of a previously uncharacterized family of enzymes acting as Baeyer–Villiger monooxygenases.