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Enzymes from the Haloacid Dehalogenase (HAD) Superfamily Catalyse the Elusive Dephosphorylation Step of Riboflavin Biosynthesis
Author(s) -
Haase Ilka,
Sarge Sonja,
Illarionov Boris,
Laudert Dietmar,
Hohmann HansPeter,
Bacher Adelbert,
Fischer Markus
Publication year - 2013
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201300544
Subject(s) - dephosphorylation , biosynthesis , superfamily , dehalogenase , biochemistry , riboflavin , escherichia coli , enzyme , chloroplast , computational biology , chemistry , biology , gene , phosphatase
The missing link: Studies on the biosynthesis of riboflavin have failed to characterise dephosphorylation of the intermediate 5‐amino‐6‐ribitylamino‐2,4(1 H ,3 H )‐pyrimidinedione 5′‐phosphate. We show that this reaction can be catalysed in Escherichia coli by YigB and YbjI and in plant chloroplasts by AtcpFHy1, which are members of the haloacid dehalogenase superfamily.