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Cell‐Free Preparation of Functional and Triggerable Giant Proteoliposomes
Author(s) -
Liu YanJun,
Hansen Gregory P. R.,
VenancioMarques Anna,
Baigl Damien
Publication year - 2013
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201300501
Subject(s) - liposome , membrane , membrane protein , cell , chemistry , cell membrane , function (biology) , biophysics , microbiology and biotechnology , nanotechnology , materials science , biochemistry , biology
Heat, we leak: We express a membrane protein outside well‐defined giant liposomes obtained by gravity‐transferred sucrose‐in‐oil droplets into a cell‐free, reconstituted expression system. We show that the presence of the liposome is necessary during expression for efficient protein insertion into the membrane and that temperature can trigger the resulting membrane function.