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A Fluorogenic Probe for γ‐Glutamyl Cyclotransferase: Application of an Enzyme‐Triggered O‐to‐N Acyl Migration‐Type Reaction
Author(s) -
Yoshiya Taku,
Tsuda Shugo,
Mochizuki Masayoshi,
Hidaka Koushi,
Tsuda Yuko,
Kiso Yoshiaki,
Kageyama Susumu,
Ii Hiromi,
Yoshiki Tatsuhiro,
Nishiuchi Yuji
Publication year - 2013
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201300481
Subject(s) - dipeptide , enzyme , substrate (aquarium) , chemistry , biochemistry , glutathione , substrate specificity , chemical biology , stereochemistry , biology , amino acid , ecology
Light it up: Human chromosome 7 ORF 24, a tumor‐related protein, has been identified as a γ‐glutamyl cyclotransferase (GGCT) in the glutathione homeostasis cycle. The singular substrate preference of the enzyme has hampered chemical probe development, and no fluorogenic probe has been reported. Here we report the first fluorogenic dipeptide probe, LISA‐4, which should contribute toward further understanding of GGCT.