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Time‐Resolved in‐Situ Observation of Starch Polysaccharide Degradation Pathways
Author(s) -
Beeren Sophie R.,
Petersen Bent O.,
Bøjstrup Marie,
Hindsgaul Ole,
Meier Sebastian
Publication year - 2013
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201300461
Subject(s) - amylopectin , starch , chemistry , in situ , polysaccharide , heteronuclear single quantum coherence spectroscopy , nuclear magnetic resonance spectroscopy , biochemistry , amylose , organic chemistry
Analytical challenges in the direct time‐resolved observation of starch metabolism have been addressed by using optimized multidimensional NMR experiments. Starch provides the main source of human dietary energy intake and is a raw material for beverage and renewable fuel production. Use of direct in situ observations of starch remodeling pathways could facilitate our understanding and control of processes of biotechnological, medical, and environmental relevance. Processes involving starch synthesis or degradation are difficult to monitor directly in aqueous solution, however, because starch consists of glucopyranosyl homopolymers that are built up from and degraded into structurally similar fragments that yield only small signal dispersion in optical and NMR spectroscopy. By focusing on acetal groups only, 1 H, 13 C HSQC experiments sampling narrow spectral windows in the highly resolved 13 C dimension have been employed in order to observe the amylopectin cleavage pathway in real time with a temporal resolution of 150 s. Quantifiable signals for more than 15 molecular species emerging during starch fragmentation by human saliva have been resolved and tracked over time in this manner. Altered accumulation of intermediates in the digestion of amylopectin in the presence of black tea acting as an effector have been monitored.