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Expanding the Library and Substrate Diversity of the Pyrrolysyl‐tRNA Synthetase to Incorporate Unnatural Amino Acids Containing Conjugated Rings
Author(s) -
Lacey Vanessa K.,
Louie Gordon V.,
Noel Joseph P.,
Wang Lei
Publication year - 2013
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201300400
Subject(s) - aminoacyl trna synthetase , transfer rna , amino acid , conjugated system , amino acyl trna synthetases , substrate specificity , chemistry , genetic code , synthetic biology , substrate (aquarium) , combinatorial chemistry , computational biology , biochemistry , biology , stereochemistry , enzyme , rna , gene , organic chemistry , ecology , polymer
Unnatural amino acids (UAAs) containing conjugated ring systems are of interest for their optical properties. Until now, such bulky and planar UAAs could not be incorporated into proteins using the pyrrolysyl tRNA/synthetase shuttling system. Using the “small‐intelligent” approach to construct a highly diverse library, we evolved novel synthetases specific for two such UAAs and incorporated them into proteins in E. coli and mammalian cells.