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An Unconventional Glycosyl Transfer Reaction: Glucansucrase GTFA Functions as an Allosyltransferase Enzyme
Author(s) -
Timm Malte,
Görl Julian,
Kraus Michael,
Kralj Slavko,
Hellmuth Hendrik,
Beine Raphael,
Buchholz Klaus,
Dijkhuizen Lubbert,
Seibel Jürgen
Publication year - 2013
Publication title -
chembiochem
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.05
H-Index - 126
eISSN - 1439-7633
pISSN - 1439-4227
DOI - 10.1002/cbic.201300392
Subject(s) - lactobacillus reuteri , chemistry , enzyme , biochemistry , computer science , stereochemistry , lactobacillus , fermentation
First reported allosyltransferase: The allopyranosyl analogue of sucrose (All‐Fru) was used as donor for the glucansucrase GTFA from Lactobacillus reuteri 121. This enzyme is able to hydrolyse All‐Fru and transfer allose to acceptor substrates such as sugars, amino acids and (−)‐epicatechin, thus forming artificial α‐linked allopyranosyl products. A triple GTFA mutant showed twofold increase in allosyl transfer.